منابع مشابه
Comparison of the reflectance and Crystal Violet assays for measurement of biofilm formation by Enterococcus
In previous work, we described a protocol for measuring biofilm formation on opaque and non-opaque surfaces using reflected light. In the present work, we used the reflectance assay to assess biofilm formation for 14 strains of Enterococcus and compared our results with those obtained using a Crystal Violet (CV) assay. In general, the results for the two assays were in agreement: both identifie...
متن کاملQuantitative adsorption of crystal violet.
The formula for crystal violet given in Conn's book (1940) implies that quantitative measurement of the dye adsorbed by bacterial cells should be possible by a suitable micro-Kjeldahl procedure. At the present time there is no satisfactory method for estimating the degree of gram positivity although the need for such a procedure is recognized and is discussed by Bartholomew and Mittwer (1952). ...
متن کاملEvaluation of MTT and Trypan Blue assays for radiation-induced cell viability test in HepG2 cells
Background: Cell viability is an important factor in radiation therapy and thus is a method to quantify the effect of the therapy. Materials and Methods: The viability of human hepatoma (HepG2) cells exposed to radiation was evaluated by both the MTT and Trypan blue assays. The cells were seeded on 96 well-plates at a density of 1 x 104 cells/well, incubated overnight, and irradiated with...
متن کاملCrystal violet reactions of coagulase negative staphylococci.
Twenty four reference strains and 112 clinical isolates of coagulase negative staphylococci (CNS) were examined for their reactions in the crystal violet test. Some species gave a white reaction and others a purple reaction. Results were consistent and reproducible and each species gave only one pattern of crystal violet reaction. Within the limited variety of species represented in the clinica...
متن کاملThe Comparison of MTT and CVS Assays for the Assessment of Anticancer Agent Interactions
Multiple in vitro tests are widely applied to assess the anticancer activity of new compounds, including their combinations and interactions with other drugs. The MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) assay is one of the most commonly used assays to assess the efficacy and interactions of anticancer agents. However, it can be significantly influenced by compounds t...
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ژورنال
عنوان ژورنال: International Journal of Toxicology
سال: 2016
ISSN: 1091-5818,1092-874X
DOI: 10.1177/1091581816648906